Development and validation of a high-throughput qPCR platform for the detection of soil-transmitted helminth infections
Resulting from the rigorous developmental approach taken, the platform we describe here provides the needed confidence in testing outcomes when utilized in conjunction with large-scale efforts such as the DeWorm3 trial. Additionally, the successful development of an evaluation and validation strategy provides a template for the creation of similar diagnostic platforms for other neglected tropical diseases.
Authors
- S. Manigandan (ORCID: https://orcid.org/0000-0003-2039-3393)
- Malathi Manuel (ORCID: https://orcid.org/0000-0002-2575-9721)
- Jaco J. Verweij (ORCID: https://orcid.org/0000-0001-8553-931X)
- Alan J. Warren (ORCID: https://orcid.org/0000-0001-9277-4553)
- Nils Pilotte (ORCID: https://orcid.org/0000-0002-8447-7425)
- Judd L. Walson (ORCID: https://orcid.org/0000-0003-4836-720X)
- Victor Omballa (ORCID: https://orcid.org/0000-0003-2984-3012)
- Andrew Gonzalez (ORCID: https://orcid.org/0000-0001-6075-8081)
- Steven A. Williams (ORCID: https://orcid.org/0000-0002-4881-7496)
- Ushashi C. Dadwal (ORCID: https://orcid.org/0000-0002-8610-4825)
- Moudachirou Ibikounlé (ORCID: https://orcid.org/0000-0002-3517-1729)
- Jeanne Goodman
- Sitara Swarna Rao Ajjampur (ORCID: https://orcid.org/0000-0003-3419-6577)
- Sean R. Galagan
- Tim Littlewood
- Leah R. Padgett (ORCID: https://orcid.org/0000-0001-9295-9689)
- Zayina Zondervenni Manoharan
- Ajith Kumar Muthukumar
- Lisette van Lieshout
- Gretchen Walch
- Doug Rains
- M. K. Sheikh
- Monica Voss
Institutions
- Natural History Museum (GB)
- Johns Hopkins University (US)
- Christian Medical College, Vellore (IN)
- University of Washington (US)
- Smith College (US)
- Leiden University Medical Center (NL)
- Université d'Abomey-Calavi (BJ)
- Elisabeth-TweeSteden Ziekenhuis (NL)
- Quinnipiac University (US)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2025-01-10
- DOI
- https://doi.org/10.1371/journal.pntd.0012760
- Citations
- 4
- Primary Topic
- Parasites and Host Interactions
- Type
- article
- Field-Weighted Citation Impact
- 8.19
Funders
- Bill and Melinda Gates Foundation
- University of Washington
- Christian Medical College, Vellore