Cytocompatible and antibacterial efficacy of surface designed Ti40Cu40Zr11Fe3Sn3Ag3 amorphous alloy for potential biomedical applications

This study explores the feasibility of utilizing chemically treated Ti 40 Cu 40 Zr 11 Fe 3 Sn 3 Ag 3 at% amorphous alloy as a potential implant material. This work involves synthesis of nano-structured surfaces using chemical pseudo-dealloying by employing an ammonia-based solution (NH 4 OH and H 2 O 2 ). Two distinct surfaces were developed by modifying solution concentration and in vitro cytocompatibility and antibacterial response of the treated ribbon samples were assessed. The findings reveal that the modified Ti-based amorphous alloy is cytocompatible with Saos-2 and HOb cells and exhibits antibacterial activity against the clinically relevant gram-negative pathogen Pseudomonas aeruginosa . Moreover, the modified surfaces can induce the release of reactive oxygen species, such as hydroxyl radicals, in a cell-free medium simulating an inflammatory system, suggesting a potential antibacterial response. • Ti-based alloy surfaces exhibit antibacterial effects against P. aeruginosa . • Pseudo-dealloying creates nanoporous surfaces for effective biofilm inhibition. • DS-60min sample enhances ROS generation and reduces bacterial adhesion. • Cytocompatible with Saos-2 and HOb cells, ensuring safe implant potential. • Modified surfaces offer promising prospects for antimicrobial biomaterials