Noval Selective Ligand‐Extraction of Bioactive Components in Complex Natural Products Applying Immobilized Multi‐Target Magnetic Beads
Choerospondias axillaris is a medicinal plant used for treating coronary heart disease (CHD) due to its broad spectrum of anti-inflammatory activities. Cyclooxygenase 2 (COX-2) and lipoxygenase 5 (5-LOX) were immobilized on magnetic nanoparticles for selective ligand-extraction of these two enzymes present in C. axillaris. Sixteen extracted components were identified and analyzed using ultraperformance liquid chromatography plus Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-ExactiveOrbitrap-MS/MS), including flavonoids Curcumin and Epicatechin. The metabolization of the aforementioned 16 components in rats with acute blood stasis indicated a solid pharmacodynamic foundation. This method offers an effective approach for conducting basic research on the enrichment, analysis, and efficacy evaluation of active components in complex natural products.
Authors
- Xun Gao (ORCID: https://orcid.org/0000-0002-7339-523X)
- Shuai Sun (ORCID: https://orcid.org/0000-0003-4953-9288)
- Hongsen Wang (ORCID: https://orcid.org/0000-0002-8328-4410)
- Xiao Dai
- Kunming Qin (ORCID: https://orcid.org/0000-0001-8463-1608)
- Chen Wang (ORCID: https://orcid.org/0000-0002-3951-3159)
- Ying Wang (ORCID: https://orcid.org/0000-0003-0296-7402)
- Yiran Li (ORCID: https://orcid.org/0000-0001-7922-7766)
- Weiping Wang (ORCID: https://orcid.org/0000-0001-7511-3497)
- Xueying Qin
- Xianhong Zhu
- Qing Zhang (ORCID: https://orcid.org/0000-0003-1473-0592)
Institutions
- Nanjing University of Chinese Medicine (CN)
Publication Details
- Journal
- Biomedical Chromatography
- Published
- 2025-01-09
- DOI
- https://doi.org/10.1002/bmc.6060
- Citations
- 1
- Primary Topic
- Phytochemicals and Antioxidant Activities
- Type
- article
- Field-Weighted Citation Impact
- 0.77
Funders
- National Natural Science Foundation of China
- Priority Academic Program Development of Jiangsu Higher Education Institutions