Diselenides as novel effective fluorescence quenchers to construct a two-photon fluorescent probe for thiols in a mouse stroke model
A fluorescence quenching mechanism using linear diselenides was proposed for the first time through a combination of intramolecular charge transfer (ICT) and Förster resonance energy transfer (FRET). Herein, we synthesized and screened a two-photon fluorescent probe AFC-SeSe, demonstrating a remarkable 300-fold increase in response to glutathione (GSH). Additionally, AFC-SeSe enabled real-time observation of increased thiol levels following treatment within a short timeframe in a mouse model of stroke.
Authors
- Jintao Zhao (ORCID: https://orcid.org/0000-0002-7328-5401)
- Baoxin Zhang (ORCID: https://orcid.org/0000-0003-2854-6796)
- Jianguo Fang (ORCID: https://orcid.org/0000-0002-2884-3363)
- Shengxiang Zhang (ORCID: https://orcid.org/0000-0001-6524-9673)
- Bingbing Chang (ORCID: https://orcid.org/0000-0002-7198-6060)
- Zhibin Zhang (ORCID: https://orcid.org/0000-0001-8759-3555)
- Tao Ma (ORCID: https://orcid.org/0000-0003-1188-6620)
- Hao Gao (ORCID: https://orcid.org/0009-0007-5947-1295)
- Jun Wu (ORCID: https://orcid.org/0009-0009-9202-3034)
Institutions
- Nanjing University of Science and Technology (CN)
- Lanzhou University (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d4cc06286h
- Citations
- 9
- Primary Topic
- Sulfur Compounds in Biology
- Type
- article
- Field-Weighted Citation Impact
- 10.01
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Gansu Province
- Science and Technology Department of Gansu Province