Determining the Collagen Content and Concentration of Nanofat and Microfat

This study investigates the collagen content and concentration in Microfat and Nanofat using SDS-PAGE and Sirius Red Dye (SRD) assay analysis. Microfat consists of small clumps of fat derived from lipoaspirate, while Nanofat is a broken-down lipoaspirate with no adipose cells but rich in stromal vascular fractions. The hypothesis posits that Nanofat, devoid of adipocytes, would contain higher collagen concentrations.