Two-factor ANOVA Method for the Determination of Sb in Glass Beads used for Pavement Marking via HG-HRCS-AAS

In recent years, the demand for glass beads for road marking is increasing, but the problem of low detection accuracy of heavy metal content in glass bead is prominent. Based on this background, this paper proposes an accurate and sensitive two-factor analysis of variance (Two-factor ANOVA) method to determine the content of Sb in glass beads by high-resolution continuous light source atomic absorption spectrometry (HG-HRCS-AAS).Firstly, the sample pretreatment conditions are optimized through orthogonal test design to determine the best digestion ratio of glass bead samples, and the combination of the best digestion temperature, holding time and heating time is obtained by combining range analysis and variance analysis methods. The best acid removal temperature and holding time are determined by the test results. Secondly, we use the two-factor ANOVA to optimize the instrument conditions and reaction conditions. The former includes sensitivity conditions, atomization conditions, and injection conditions. Another includes pre-reduction conditions and hydride generation conditions. Finally,using this method to analyze Sb standard material and get the following results: the relative standard deviation (RSD) of Sb in the glass bead sample was 1.33% and the Limit of detection(LOD) was 0.147μg/L, limit of quantit-ation(LOQ) is 0.490μg/L, the recovery rate of blank spiking is 96%-104%.