Excessive consumption of -solanine poisons the trophoblast via increasing the concentrations of autophagic marker which induces cell autophagy

There exists a large amount of toxins in the surrounding environment which have the capacity to damage our body's health. -Solanine, a glycoalkaloid ordinarily extracted from potatoes, possesses potential toxicity to the human body, which may induce cell apoptosis and autophagy. The trophoblast, an embryonic tissue, is crucial in human development. However, how the toxin -solanine impacts the trophoblast still remains unclear. This endangers many pregnant women and the coming generation nowadays. Hence, this study is aimed at exploring the mechanism of trophoblast poisoning triggered by excessive consumption of -solanine via varying the concentrations of two proteins, Beclin-1 and LC3, which exert crucial roles in the cell autophagy signaling pathway, using HTR-8/SVneo cells as the sample. By conducting IHC analysis and autophagy staining assay kit with monodansylcadaverine (MDC), the changed concentrations of Beclin-1, LC3 and autophagosome have a relationship between the increasing concentration and duration of -solanine. Meanwhile, conducting the cell proliferation experiment offers a bidirectional confirmation of the aforementioned results. In conclusion, the mechanism of poisoning is that the excessive consumption of -solanine promotes the synthesis of Beclin-1 and LC3, which directly stimulates the progress of cell autophagy and decreases cell viability. Therefore, these ultimately cause detriment to the trophoblast.