Use ER stress inhibitor BiP Inducer X in ZIKV-infected brain organoid to see if reducing ER stress through ATF6 pathway can alleviate pathological feature of Alzheimers disease

Alzheimers disease (AD), a gradually worsening neurodegenerative disorder, typically affects the elderly people. Widely acknowledged as the primary cause of AD is the accumulation of -amyloid (A) outside cells and the formation of neurofibrillary tangles inside cells. Presently, there exists no efficacious therapy to slow down the emergence and progression of AD and many scientists are finding ways to cure AD through different aspects. There are some experiments have indicated that ER stress contributes to the onset and development of AD. Therefore, some scientists are having experiments on AD by inhibit ER stress using different inhibitors through different pathways. Zika virus (ZIKV) have been proved to be able to increase a accumulation and p-tau level in AD brain organoid. After ZIKV infection, phenotypes linked to AD in brain organoids saw an increase due to stress in the endoplasmic reticulum (ER) and the unfolded protein response (UPR).The BiP Inducer X(BIX)is used in this experiment to investigate the effect of inhibitor on AD via activating transcription factor 6(ATF6)pathway in ZIKV-infected brain organoid models. The A accumulation and p-tau level is recorded by immunostaining to form image. This paper only provides theoretical experiment design and possible result about whether the inhibition on ER stress through ATF6 pathway would reduce AD features, which need further research in the pathology of AD.