Polymorphism detection of catalase-21A\T (rs 7943316) gene in chronic myeloid leukemia patients infected with human herpes virus-7

Abstract: BACKGROUND: Chronic myeloid leukemia (CML) is a type of cancer that originates in hematopoietic stem cells, particularly those that develop into myeloid cells. A key feature of CML is the Philadelphia (Ph) chromosome, which is produced by a reciprocal translocation between chromosome 9 and chromosome 22 (designated as t [9;22]). This chromosomal change results in the creation of the BCR-ABL1 fusion gene. Several genetic association studies have analyzed the link between the catalase (CAT) C262T variant and different cancers, but the findings remain controversial. OBJECTIVE: This study was aimed to determine the association between CAT-21A\T (rs 7943316) gene polymorphism and the percentage of human herps virus-7 (HHV-7) in patients with CML. PATIENTS AND METHODS: A case–control study included 120 CML blood specimens enrolled in the current research, including 40 newly diagnosed CML patients and 80 treated CML patients, as well as 50 blood specimens collected from persons as the control group. Sequencing was used to identify the CAT-21A\T (rs 7943316) gene polymorphism and HHV-7 DNA using conventional polymerase chain reaction (PCR). RESULTS: The current study included 40 newly diagnosed CML patients with a mean age of 48 ± 12.7 years and 80 treated CML patients with a mean age of 50 ± 10.53 years, whereas the mean age of the 50 controls was 50 ± 12.95 years. PCR testing for HHV-7 revealed positive results in 6 (15%) of newly diagnosed CML patients and in 19 (23.8%) of those treated for CML. In contrast, one person in the control group was infected by HHV-7. The difference in frequency of genotype distribution of the polymorphism between newly diagnosed, treated patients with CML and control groups was statistically significant. There was no correlation between CAT-21A\T (rs 7943316) polymorphism and HHV-7 infection. New recording for CAT-21A\T (rs 7943316) polymorphism in gene bank NCBI and American bank. CONCLUSION: HHV-7 acts as a cofactor in pathogenesis as well as the development biology of CML in Iraqi patients. Polymorphism of CAT-21A\T (rs 7943316) may play a role as a risky factor in the pathogenesis of idiopathic CML.