Effects of Resveratrol on the Kidney in Rats with Streptozotocin Induced Diabetic Nephropathy

Objective: Diabetic nephropathy is one of the most significant causes of end-stage renal failure and is a common microvascular complication of diabetes (D).Resveratrol (RSV), a natural compound found in grape skins and red wine, has potent antioxidant properties.This study aimed to evaluate the effects of RSV in a streptozotocin (STZ)-induced diabetic rat model. Methods:Animals were divided into four groups: control, RSV, D, and D + RSV.The diabetic group received a single intraperitoneal dose of STZ (65 mg/kg).After 2 weeks, rats with basal blood glucose levels >250 mg/dL were considered diabetic.RSV(10 mg/kg/day) was administered orally by gavage for 8 weeks.Metabolic analyses were conducted throughout the study.At the study's end, transmission electron microscopy and immunohistochemical analyses were performed.Additionally, the left kidney was isolated and suspended in an organ bath to study the functional changes without damaging the renal artery.Results: In the study, increased transforming growth factor-beta, fibronectin and inducible nitric oxide synthase immunoreactivity, which are markers of D-induced renal degeneration, were partially Ama: Diyabetik nefropati, son dnem bbrek yetmezliinin en nemli nedenlerinden biridir ve diyabetin (D) yaygn bir mikrovaskler komplikasyonudur.zm kabuu ve krmz arapta bulunan doal bir bileik olan resveratrol (RSV), gl antioksidan zellikler gsterir.Bu almada, streptozotosin ile oluturulan D san modelinde RSV'in diyabetik nefropati zerindeki etkilerinin deerlendirilmesi amalanmtr.Yntemler: Hayvanlar kontrol, RSV, D ve D + RSV olmak zere drt gruba ayrld.D gruplara tek bir intraperitoneal doz streptozotosin (65 mg/kg) uyguland.ki hafta sonra, bazal kan glukoz seviyeleri 250 mg/ dL'nin zerinde olan sanlar D olarak kabul edildi.RSV (10 mg/kg/gn) 8 hafta boyunca gavaj yoluyla oral olarak uyguland.alma boyunca metabolik analizler yapld.almann sonunda, transmisyon elektron mikroskopisi ve immnohistokimyasal analizler yapld.Ek olarak, sol bbrek izole edildi ve renal artere zarar vermeden fonksiyonel deiiklikleri incelemek iin bir organ banyosunda askya alnd.