Negative Geotaxis Assay in Three Drosophila Strains Consuming Bisphenol A: Duration and Number of Climbing Successes

Abstract Bisphenol A (BPA) is a substance that can migrate from polycarbonate plastic packaging to food or beverages. Several countries have banned the use of BPA in various food packaging, while other countries still have looser regulations. This study aims to analyze the effect of BPA consumption and strain type on the climbing performance of Drosophila melanogaster . Negative geotaxis assay was conducted on 3 groups of Drosophila : without BPA, consumed 0.25 mg/mL BPA, and consumed 0.50 mg/mL BPA. In the climbing duration, BPA did not have a significant effect (F = 1.682, p = 0.240), strain had a significant effect (F = 8.807, p = 0.008), and the interaction between the two did not have a significant effect (F = 0.267, p = 0.892). In the climbing success, BPA consumption had a significant effect (F = 9.600, p = 0.006), strain had no significant effect (F = 4.200, p = 0.051), while there was a significant interaction between the two factors (F = 12.900, p = 0.001). Therefore, BPA consumption can interfere with the motor skills of Drosophila imago. This study implies that BPA exposure has the potential to be a risk factor for motor disorders in higher organisms, including humans.