Single-cycle parainfluenza virus type 5 vectors for producing recombinant proteins, including a humanized anti-V5 tag antibody
Parainfluenza virus type 5 (PIV5) can cause either persistent or acute/lytic infections in a wide range of mammalian tissue culture cells. Here, we have generated PIV5 fusion (F)-expressing helper cell lines that support the replication of F-deleted viruses. As proof of the principle that F-deleted single-cycle infectious viruses can be used as safe and efficient expression vectors, we have cloned and expressed a humanized (Hu) version of the mouse anti-V5 tag antibody (clone SV5-Pk1). We show that multiple different cell lines can be infected and express high levels of the Hu anti-V5 antibody, with Chinese hamster ovary cells expressing 20-50 mg l<sup>-1</sup> after 5 days when cells were grown to a density of ~1×10<sup>6</sup> cells per millilitre at the time of infection. We suggest that PIV5-based vectors may be further developed to produce recombinant proteins both <i>in vitro</i> and <i>in vivo</i>.
Authors
- J. Andrejeva
- Stephen Goodbourn (ORCID: https://orcid.org/0000-0002-4530-1179)
- David J. Hughes (ORCID: https://orcid.org/0000-0003-1668-8770)
- Volker Morath (ORCID: https://orcid.org/0000-0001-7636-0537)
- L.J. West
- Richard E. Randall (ORCID: https://orcid.org/0000-0002-9304-6678)
- D. F. Young (ORCID: https://orcid.org/0000-0003-4972-1311)
- Maria Pisliakova
- Luis Rossler
Institutions
- St George's, University of London (GB)
- University of St Andrews (GB)
- TUM Klinikum (DE)
- Technical University of Munich (DE)
Publication Details
- Journal
- Journal of General Virology
- Published
- 2025-01-09
- DOI
- https://doi.org/10.1099/jgv.0.002061
- Citations
- 1
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 1.35
Funders
- Medical Research Charities Group
- University of St Andrews
- Medical Research Council