A dynamical model towards continuous single-cell cultivation using photobioreactors

Phenomenological models for different stages of a photosynthetic system have helped predict experimental outcomes at the cell level. Similar models exist to predict process outcomes at the bioreactor scale. However, biological and process models are typically ontologically separate, as process models do not include explicit mappings of cellular processes. This work presents a model for the dynamics of photobioreactors with phenomenological reaction kinetics. The model allows for design and control integration, with insights into design parameters and operating conditions aiding both the growth of microalgae and the production of valuable lipids. The model also enables integration of process topology such that the reactor network connections yielding a complete process can be modelled alongside reaction kinetics. Therefore, we provide insights on nonlinear dependence of biomass and lipid production using microalgae cultivation, design parameters such as incident light intensity and photobioreactor radius, and process operating conditions such as flow rates. • A dynamical model for continuous single-cell cultivation is presented. • Dynamical model considers light and flow rate of species into the reactor as only inputs. • Dependence on radial design parameter is also studied to yield optimal conditions for growth and production. • Simplicity of the model could aid in control and monitoring applications.