Fluorescence immunochromatographic assay for deoxynivalenol using immunomagnetic bead purification

Deoxynivalenol (DON) contaminates various complex matrices, necessitating straightforward, effective cleanup and precise detection methods. This study employed immunomagnetic beads for sample purification and utilized a competitive time-resolved fluoro-immuno-chromatographic assay to achieve quantitative detection of DON in corn and its by-products. The limits of detection and quantification were 104 μg/kg and 243 μg/kg, respectively. Significant cross-reactivity was absent with most common toxins, except for 3-acetyl-deoxynivalenol, which exhibited a cross-reaction rate of 3167%. The recovery rates ranged from 86% to 117%, with coefficients of variation between 6.9% and 9.5%. The correlation coefficient with HPLC was 0.977. This method is rapid, accurate, and requires no large-scale equipment, facilitating on-site detection directly.