Characterization of the complete chloroplast genome of medicinal species Duhaldea nervosa (Asteroideae, Asteraceae) and its phylogenetic analysis

Here, we de novo assembled and analyzed the chloroplast (cp) genome of Duhaldea nervosa (Asteroideae, Asteraceae) that showed a quadripartite structure with a total length of 150,916 base pairs (bp). It consisted of a pair of inverted repeats (IRa and IRa each of 24,927 bp), which separated the small single copy (18,188 bp) and large single copy (82,874 bp) regions. The genome contained 112 unique genes, including 4 ribosomal RNA (rRNA), 79 protein-coding genes (CDS), and 29 transfer RNA (tRNA) genes. Phylogenetic analysis among 79 species revealed a sister relationship between D. nervosa and Duhaldea cappa, which lay within the tribe Inuleae. At the tribal level, our phylogenetic findings indicated a sister relationship of “Astereae with Anthemideae” and “Gnaphalieae with Calenduleae,” and rooted by Senecioneae. This study enhanced the understanding of cp genome evolution and phylogeny within the Asteraceae family.