In silico Identification of Putative Novel Therapeutic Targets in Xanthomonas campestris Pv. Campestris to Combat Black Rot Disease in Brassica oleracea

Background: Xanthomonas campestris pv. campestris, the plant pathogenic bacteria causing black rot is a threat in the agriculture of Brassica oleracea crops. It is one of the most economically concerning diseases in the agriculture of cabbages. Effective compete solutions to combat the disease are still lacking. Methods: In the quest to find an effective solution to combat the disease, an in silico, subtractive genomic and proteomic approach was adopted. Various bioinformatics tools, online databases and servers were used in this study to find the best therapeutic targets in the proteome of the bacteria. Result: Seven novel targets were filtered out of six hundred and three essential proteins by multi-step subtractive proteomic approaches. Homology and uniqueness were duly checked to prevent any adverse effects in the host plant while using any inhibitory molecule against these seven targets.