Histopathological Alterations in Cutaneous Wound Healing and Pancreatic Architecture in Alloxan-Induced Diabetic Rabbits

The study evaluated the pancreatic cellular responses and histopathological changes associated with the cutaneous wound healing g of alloxan induced diabetic New Zealand White (NZW). Sixteen adult rabbits of either sex, weighing 1.8 – 3.2 kg were used for the study. They were divided into four group. s Group A is the control, it was -non diabetic and no wound. Group B was diabetic and no wound, whereas group C was non-diabetic and with wounded while group D was having wounded and diabetic. Diabetes induction was accomplished by administration of 100 mg/kg of alloxan monohydrate twice, 72 h apart in groups B and D only. A 3 cm 2 excisional skin wound was created at the dorsum of each of the rabbits in groups C and D. Skin tissue samples (wounded sites) and pancreas were harvested from two euthanized rabbits in each group on day 28 post induction of diabetes for histopathologic examinations. Diabetes was induced in groups B and D on day 3 post treatment. Histopathologic findings in the diabetic rabbits included hyperkeratosis, acanthosis, poor fibrogenesis of the injured skin site, as well as the necrosis and mononuclear cellular infiltration of the islet of Langerhans of the pancreas. Conclusion diabetes mellitus caused delayed skin wound healing up to day 28 post wound creation, evidenced by hyper cellularity and fibrous tissue proliferation pancreatic necrosis and cellular infiltration of the islets of Langerhans on day in the diabetic rabbit.