Attenuation of alginate lyase by chlorhexidine in the alginate pathway of Pseudomonas aeruginosa biofilm formation

Ventilator-associated pneumonia (VAP) caused by Pseudomonas aeruginosa poses a significant clinical challenge due to the pathogen's robust biofilm formation and resistance mechanisms. Chlorhexidine (CHX), a commonly used antiseptic, has shown potential in biofilm inhibition, though its exact mechanism remains unclear. Our previous work focusing on quorum sensing associated proteins identified strong CHX binding to PslG, a key protein in the Psl pathway. However, the potential for CHX interac