MOLECULAR-BIOLOGICAL METHODS OF LABORATORY DIAGNOSTICS

The lecture highlights the fundamental concepts of molecular diagnostics. It presents insights into modern molecular-biological methods of laboratory diagnostics, emphasizing their role in detecting infectious agents, and gene mutations, and assessing hereditary disease risks. The material provides an in-depth overview of polymerase chain reaction (PCR) principles, DNA sequencing, hybridization techniques, and isoelectric focusing. Special attention is given to the advantages, challenges, and potential of these methods in clinical practice. Furthermore, the lecture discusses how molecular diagnostics supports personalized treatment approaches and emphasizes the importance of innovative equipment for effective pathology diagnostics.