Society for Immunotherapy of Cancer: updates and best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) image analysis and data sharing
mIHC/IF technologies are maturing and are routinely included in research studies and moving towards clinical use. Guidelines for how to perform and standardize image analysis on mIHC/IF-stained slides will likely contribute to more comparable results across laboratories and pave the way for clinical implementation. A checklist encompassing these two-part guidelines for the generation of robust data from quantitative mIHC/IF assays will be provided in a third publication from this task force. While the current effort is mainly focused on best practices for characterizing the tumor microenvironment, these principles are broadly applicable to any mIHC/IF assay and associated image analysis.
Authors
- Shirley Greenbaum (ORCID: https://orcid.org/0000-0002-0680-7652)
- Michael R. Angelo (ORCID: https://orcid.org/0000-0003-1531-5067)
- Jaime Rodriguez‐Canales (ORCID: https://orcid.org/0000-0002-0885-2377)
- Jeffrey S Roskes
- Jennifer H. Yearley
- Alexander S. Szalay (ORCID: https://orcid.org/0000-0002-4108-3282)
- Joel Sunshine (ORCID: https://orcid.org/0000-0001-9987-6712)
- David L. Rimm (ORCID: https://orcid.org/0000-0001-5820-4397)
- Ignacio I. Wistuba (ORCID: https://orcid.org/0000-0003-3365-6340)
- Emanuel Schenck
- Elizabeth L. Engle (ORCID: https://orcid.org/0000-0002-6375-9060)
- Janis M. Taube (ORCID: https://orcid.org/0000-0002-8273-4395)
- Kurt A. Schalper (ORCID: https://orcid.org/0000-0001-5692-4833)
- Edwin R. Parra (ORCID: https://orcid.org/0000-0001-9068-1636)
- Margaret Eminizer (ORCID: https://orcid.org/0000-0003-4591-2225)
- Ana Lako (ORCID: https://orcid.org/0000-0003-2931-6212)
- Travis J. Hollmann (ORCID: https://orcid.org/0000-0003-1599-0433)
- Scott J. Rodig (ORCID: https://orcid.org/0000-0003-1761-9769)
- Noah F. Greenwald (ORCID: https://orcid.org/0000-0002-7836-4379)
- Carlo Bifulco (ORCID: https://orcid.org/0000-0003-1922-6977)
- Michael T. Tetzlaff (ORCID: https://orcid.org/0000-0001-7899-4138)
- Michael Surace (ORCID: https://orcid.org/0009-0000-8326-5159)
- Cyrus V. Hedvat (ORCID: https://orcid.org/0000-0003-0045-3491)
- Sacha Gnjatic (ORCID: https://orcid.org/0000-0001-5643-9520)
- Daniel Jiménez‐Sánchez (ORCID: https://orcid.org/0000-0002-1695-8761)
- Güray Aktürk (ORCID: https://orcid.org/0000-0001-7032-8085)
- Benjamin Green (ORCID: https://orcid.org/0000-0002-2461-1274)
- Konstanty Korski
- Keith E. Steele (ORCID: https://orcid.org/0009-0005-8113-0054)
- Marlon C. Rebelatto
- Cláudia S. Ferreira
Institutions
- Roche (Switzerland) (CH)
- Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
- Bloomberg (United States) (US)
- Memorial Sloan Kettering Cancer Center (US)
- The University of Texas MD Anderson Cancer Center (US)
- Harvard University (US)
- Johns Hopkins University (US)
- University of California, San Francisco (US)
- Providence Portland Medical Center (US)
- Foundation Center (US)
- Yale University (US)
- Roche Pharma AG (Germany) (DE)
- Tisch Hospital (US)
- AstraZeneca (United States) (US)
- Dana-Farber Brigham Cancer Center (US)
- Daiichi Sankyo (United States) (US)
- Sidney Kimmel Comprehensive Cancer Center (US)
- Stanford University (US)
- Icahn School of Medicine at Mount Sinai (US)
Publication Details
- Journal
- Journal for ImmunoTherapy of Cancer
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1136/jitc-2024-008875
- Citations
- 12
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 9.67
Funders
- Johns Hopkins University
- Society for Immunotherapy of Cancer
- Bloomberg~Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University