Trace DNA Recovery: Insights from Dubai Police Casework

Trace DNA represents a critical form of forensic evidence, frequently recovered from a wide variety of touched or used items. Despite its evidentiary value, trace DNA analysis poses significant challenges due to the minute quantities of DNA involved, as well as the influence of factors such as surface type, collection methods, and environmental exposure. This study systematically examines the success rates and characteristics of trace DNA profiles recovered from six-item categories—tools, stolen items, wearable items, packaging materials, vehicles, and touched items—processed between 2021 and 2023 by the Biology and DNA Section of the Dubai Police Force. A total of 6277 cases were analyzed, encompassing a range of crimes, including homicide, suicide, missing persons, paternity disputes, and burglary. The results demonstrated an overall trace DNA success rate of 64%, with wearable items yielding the highest success rate at 76% and packaging materials yielding the lowest at 54%. Detailed analysis of positive DNA trace samples revealed significant variability in DNA profile types across item categories. Wearable items and touched items predominantly yielded full single (FS) DNA profiles, reflecting their reliability as sources of singular and high-quality DNA. Conversely, stolen items and packaging materials showed a greater prevalence of full mixed (FM) DNA profiles, highlighting their association with complex mixtures due to handling by multiple contributors. Tools and vehicles, meanwhile, exhibited higher rates of partial profiles, presenting unique challenges related to surface irregularities and environmental factors. This study emphasizes the importance of tailoring forensic strategies to item-specific characteristics, as well as the need for systematic mechanisms to categorize trace samples. Addressing operational challenges such as manual sorting and leveraging automation or AI-based systems can further streamline trace DNA analysis. The findings also underscore the importance of data sharing and standardization across forensic laboratories to enhance trace DNA recovery protocols and improve reliability in forensic investigations. Future research should focus on the effects of material properties, environmental exposure, and collection techniques on DNA retention, advancing the field of trace DNA profiling and its applications in forensic science.