<i>Angelica gigas</i> Nakai Attenuates Mast Cell-mediated Inflammation via Suppression of Caspase-1 Activation

Objectives: Natural products are crucial sources for drug development due to their diverse biological properties.Angelica gigas Nakai (AG) has been traditionally used as an effective herbal medicine for diseases treatment, but its accurate anti-inflammatory mechanism is not well understood.The study aimed to investigate the mechanisms of action of AG on mast cell-mediated inflammatory responses.Methods: The antioxidant activity of AG was assessed using 2,2-diphenyl-1-picrylhydrazy (DPPH) scavenging ability and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation scavenging.To explore AG's pharmacological mechanism on inflammation, we evaluated its impact on histamine release and interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)- production in activated human mast cells-1 (HMC-1).Additionally, the inhibitory effects of AG on activation of nuclear factor-B (NF-B) and caspase-1 were determined.Results: AG demonstrated strong DPPH and ABTS + radical scavenging activity, and significantly suppressed histamine, IL-8, IL-6, and TNF- production.Moreover, AG effectively ameliorated the activation of NF-B and caspase-1 in activated-HMC-1.Conclusion: These finding suggest that AG has potential for inflammation therapy.