Efficient Hydrolysis of Fish Parvalbumin by Marine Bacterial Protease <scp>VSP2V</scp>‐280: Allergen Removal

Parvalbumin is a major allergen in fish. However, there is currently no effective and safe way to remove this allergen from fish. In this study, protease gene VSP2V-280 of marine bacteria <i>Virgibacillus</i> sp. SP2 was cloned and expressed. The protease enzyme showed maximum activity at 50°C and pH 10.0. Ca²⁺ and Cu²⁺ promoted the enzyme. The enzyme showed good parvalbumin degradation efficiency in fish. Based on the gel analysis, when 0.3 mg/mL of parvalbumin was incubated with protease VSP2V-280 (30 U/mL) containing 1 mM Ca²⁺ for 3 h, the parvalbumin removal rate reached 97%. The enzyme was further used for parvalbumin removal from <i>Ctenopharyngodon idella</i>, <i>Pelteobagrus fulvidraco</i>, <i>Parabramis pekinensis</i>, and <i>Carassius auratus</i>. The parvalbumin removal rate reached 93% in 4 h at an enzyme dosage of 72 U/mL. The study showed the potential of VSP2V-280 to remove parvalbumin from aquatic products.