The Co-Use of Various Forms of Silver and Proteases in the Development of New Dressing Biomaterials for Wound Healing

A modern complex drug for treating purulent-necrotic wounds should have a sorption capacity, enzymatic, antimicrobial, and antioxidant activities. It is promising to create medicinal antibacterial preparations based on silver nanoparticles with the desired characteristics. They should turn to a great alternative to antibiotics in the wound healing compositions. One of the promising approaches for synthesizing silver nanoparticles is the "green synthesis" by various organisms. The paper discusses the interplay between inorganic and biogenic silver nanoparticles and protease solutions. The effect of various forms of silver on the enzyme activities of proteases in solution has been identified. Biogenic silver nanoparticles have a proven advantage over salt solutions. The mutual influence of enzymes and biocides was studied. The effect of introducing chitosan into the system was also examined. The antioxidant properties of the studied nanoparticles were determined. The biocidal activity of the different silver preparations was demonstrated. The antibacterial activity of biogenic nanoparticles was at or above the activity of chemically synthesized nanoparticles. The results obtained in this work indicate the fundamental possibility of co-immobilization of proteolytic enzyme preparations and silver nanoparticles. Biological activities are maintained throughout the synthesis process.