Comparison of parentage test for Hanwoo identity test marker combinations

The identity and parentage verification tests for Hanwoo play a crucial role not only in preventing fraudulent distribution in the market but also in generating greater profits in calf transactions by verifying the parentage of superior bloodlines. Therefore, this study secured the genotypes of 946 Hanwoo cattle using the GeneTrackTM Ver1 Genotyping Kit (GT1), based on the dinucleotide marker combination recommended by ISAG, and the GeneTrackTM Ver2 Genotyping Kit (GT2), based on a combination of tri- to hexanucleotide markers, to compare the results of paternity matches and mismatches for the same animals in the Jeonbuk region. Among the 17 individuals identified as mismatches using the Hanwoo Chip SNP genotypes, 3 animals showed the same mismatch results with SNPs using the GT1 marker combination, while 14 animals were confirmed as mismatches. In the GT2 marker combination, 16 animals showed mismatches, and 1 animal was confirmed as a mismatch. These findings highlight the necessity of verifying marker genotypes and determination errors more accurately through additional samples and the configuration of Trio samples.