Genome-wide association study identifies loci affecting resistance to Scuticociliatosis in olive flounders (Paralichthys olivaceus)

Olive flounders (Paralichthys olivaceus) hold significant economic values in the aquaculture sector of South Korea. Scuticociliatosis, caused by several parasite species, poses an important threat to the aquaculture industry, particularly in olive flounders. Developing resistance to this disease is vital for sustainable aquaculture. In this study, we performed a genome-wide association study (GWAS) to identify genetic loci associated with Scuticociliatosis resistance in olive flounders. Using a time-to-event survival data as phenotype, we analyzed genomic data to detect loci affecting the Scuticociliatosis resistance. We identified the loci associated with the disease and the positional candidate genes, including NEK7, ZNF362B, PTPRUb, COL13A1, RB1, DPYSL5, and PC. Further KEGG pathway analysis revealed significant enrichment in biological pathways such as ECM-receptor interaction, Focal adhesion, Platelet activation, Rap1 signaling, and Inositol phosphate metabolism, which are closely related with immune and inflammation responses. These findings provide insight into the genetic architecture of Scuticociliatosis resistance and can assist the development of breeding strategies for disease-resistant olive flounder.