Standardization of Micropropagation Protocol in Rose (Rosa spp.) var. Divine

The present investigation was carried out to establish a standardized sterilization protocol for explants and regenerate shoots from nodal explants. Results showed that sodium hypochlorite (1%) for 4 min., cefotaxime (0.10%) for 15 min., streptomycin (0.10%) for 15 min., carbendazim (0.20%) for 15 min. and mercuric chloride (0.10%) for 4 min were the most efficient treatment with the least contamination and highest survival rates and media containing 0.50 mg/L TDZ and 3.0 mg/L BAP favored the maximum percentage of shoot initiation (100%) with an average of 4.60 shoots per node within 8.38 days of the culture period resulting longest shoot with 1.88 cm and an average of 4.53 shoots per node within 9.00 days of the culture period resulting longest shoot with 1.86 cm after 30 days of inoculation, respectively. After first sub-culture, it was found that MS medium supplemented with TDZ (0.50 mg/L) and BAP (3.0 mg/L) achieved 100% success rate for shoot multiplication.