Moringa oleifera Reduce Lipid Vacuolization, Pyknotic Cell and Organ Enlargement in Mus musculus Infected by Plasmodium berghei

Malaria is a disease that still haunts Indonesia today. The high level of anti-malarial resistance and the severity of the disease cause high mortality in malaria cases. Previous research found that Moringa oleifera can eliminate the Plasmodium berghei parasite and is an immunomodulator in mice. But how moringa works at the cellular level is still unclear. This study aimed to analyse effect of Moringa oleifera treatment in lipid vacuolization of liver, number of pyknosis cell, and organ enlargement in Mus musculus infected by Plasmodium berghei. True experimental design use 40 mice were divided into 5 group: negative control, positive control treated by dehydroartemisinin piperaquine, group 1,2, and 3 treated by 25%, 50%, and 75% Moringa extract respectively. Parasite count was determined by blood smear with giemsa staining, and their organs were collected for histopathological analysis via hematoxylin-eosin staining. Lipid vacuolization, pyknotic cell of liver was observed under light microscope. Index of Liver, spleen, and kidney were examiden by organ weight per mice body weight using digital scale. All data tested by One-Way ANOVA. The results of the study stated that Moringa oleifera reduce lipid vacuolization and reduce index of the spleen and kidney organs. However, there were no significant difference effect of Moringa oleifera in liver index and pyknotic cell. As conclusion Moringa oleifera reduce lipid vacuolization, pyknotic cell and organ enlargement in mus musculus infected by Plasmodium berghei.