In vivo and in vitro propagation of bay leaves (Laurus nobilis) in Northern Morocco

The study explored seed germination and propagation through cutting in Laurus nobilis L. with a focus on in vitro and in vivo regeneration techniques for multiplication and sustainable conservation of diversity in the species. Seed germination was achieved through pre-soaking for 7 days, followed by cold stratification at -4 °C for 50 days. The results showed that seed imbibition greatly increased germination rates (65%), however, germination in sand (14%), and peat (12%) was less successful. Imbibed seeds had a greater seedling survival rate (40%) in peat attaining better plant height. The in vitro germination of wild Moroccan L. nobilis was accomplished by scarification of kernels with sulphuric acid (34 N). Seedlings from whole fruits had the best survival rate (33.33%), followed by kernels (25%). Micropropagation of twigs using MS media with kinetin and indole-3-acetic acid proved efficient, providing useful methods for the domestication and conservation of L. nobilis .