The Effect of Ethanolic Extract of Annona muricata L. Leaves on Cerebellum Neurons in Noise-Exposed Adult Wistar Rats

Noise is an environmental condition that we encounter in everyday life. The level of noise varies in certain environments, such as work environments, traffic on highways, noise in cafeterias, and others. This exposure can affect the coordination system in our body, such as the cerebellum. Soursop leaf extract has been widely used in traditional medicine, the antioxidant content in this extract has many health benefits. Soursop leaf extract (Annona muricata L.) can be useful as an anti-inflammatory, providing protection to nerve cells from free radicals, and other benefits. This study aims to test the effect of soursop leaf extract on neurons in the cerebellum layer. The study was conducted at the experimental animal stage. The experimental animals used were adult Wistar rats which were divided into 2 control groups and 1 treatment group. Each group consisted of 15 Wistar rats. The treatment group was given ethanol extract of soursop leaves before being exposed to noise. The results showed that there were significant differences in the density of molecular layer nerve fibers, between K- and K+ groups (p=0.000) and P (p=0.015), between K+ and P groups (p=0.015). There was a significant difference in Purkinje cell degeneration in the control and treatment groups, between K- and K+ groups (p=0.000) and P (p=0.003), between K+ and P groups (p=0.015). There was no significant difference in granular cell degeneration in the control and treatment groups, between K- and K+ groups (p=0.061) and P (p=0.838), between K+ and P (p=0.094), but the degeneration was lighter in P than K+. The conclusion of this study is that soursop leaf extract provides protection to neurons in the cerebellum from noise exposure.