Assessing Platelet-derived Growth Factor and Liver Enzyme Levels in Rats Undergoing Carbon Tetrachloride Treatment and Bile Duct Ligation

Background: Platelet-derived growth factor (PDGF) is a major mitogen for connective tissue cells and certain other cell types. Objectives: This study uses bile duct ligation (BDL) to assess PDGF levels in rats and the level of liver enzymes for two periods. Methods: The experiment was divided into four groups (15 rats in each group). A total of 60 male rats were used and were divided into groups as follows: Group 1 included male rats administered with 0.5 mL/kg of drinking water as negative control; group 2 comprised male rats administered with 0.5 mL/kg of carbon tetrachloride (CCl4) orally for one month; group 3 included male rats undergoing BDL for one week; and group 4 were male rats undergoing BDL for two weeks. At the end of the treatment period, which lasted for five weeks, male rats were sacrificed and blood samples were obtained to assess PDGF and levels of liver enzyme. Results: The results showed a significant elevation (P<0.05) in the level of PDGF along with a significant elevation (P<0.05) in the level of liver enzymes (alkaline phosphatase, alanine aminotransferase, and aspartate transferase) in rats undergoing BDL and CCl4 treatment compare to control groups. Conclusion: In our current study, we concluded that a high level of PDGF is related to liver disease, and we can consider it as an indicator of cirrhosis.