THE EFFECT OF LACTOCOCCUS LACTIS (+NIS) PROBIOTIC LYSATE ON THE EXPRESSION OF ANTI-APOPTOTIC BAX AND BCL-2 GENES IN KIDNEY CANCER CELL LINE

Background & Aims: Today, Lactococcus lactis bacteria play a key role as a probiotic in the treatment and prevention of cancer.Additionally, bacteriocins-producing strains of Lactococcus lactis are able to inhibit the growth of a wide range of bacteria, especially antibiotic-resistant strains.The aim of the present study was to investigate the effects of probiotic lysate of Lactococcus lactis (+NIS) on the expression of anti-apoptotic genes in renal cancer cell lines.Materials and Methods: After isolating and examining 60 strains of Proteus mirabilis through diagnostic and biochemical tests, Multiplex PCR was performed to identify anti-apoptotic genes in renal cancer cell lines.After culturing and preparing the supernatant of nisin-producing Lactococcus lactis (PTCC1336), its activity was examined by plating on agar.Using Real-Time PCR, the expression of AcrB gene in Proteus mirabilis strains treated with supernatant was examined.Results: After the cytotoxicity studies of this supernatant, the relative expression of bcl-2 and bax genes in kidney cancer cells was studied.Nisin prepared for Proteus mirabilis strains had a slight inhibitory effect due to its mobility and virulence factors.MTT assay analysis showed that the cell survival rate in treated cancer cells decreased significantly with the passage of time and increased concentration of Lactococcus lactis cell-free supernatant.Among the 60 Proteus mirabilis samples, 37 samples had the AcrA gene, 59 samples had the AcrB gene, and 58 samples had the Urea gene.According to the analysis of MTT test data, the half-maximal inhibitory concentration (IC50) for this supernatant was calculated to be 202.8g/ml after 72 hours.Discussion: The results showed a 52.86% decrease in bcl-2 gene expression and a 60.21% increase in bax gene expression in treated cancer cells compared to the control group.Cytotoxicity studies in this research showed that with an IC50 of 202.8 g/ml, the supernatant was able to suppress the proliferation of renal cell carcinoma (ACHN).The study demonstrated that probiotic lysate Lactococcus lactis (+NIS) affected genes related to apoptosis in renal cancer cells.These lysates were able to reduce bcl-2 gene expression and increase bax gene expression, indicating the induction of apoptosis in cancer cells.