Simulation of the Glycolytic Metabolite Concentration Profile in Mammalian Resting Skeletal Muscles

A mathematical model of glycolysis in mammalian skeletal muscles is presented for the first time, in which stationary concentrations of intermediate metabolites of glycolysis are in good agreement with experimental data obtained in resting muscles. The correspondence between model and experimental values of metabolite concentrations was achieved due to the increased inhibitory effect of ATP on pyruvate kinase and a significant decrease in the [NAD+]/[NADH] concentration ratio in the cytoplasm of skeletal muscles. At the same time, activation of muscle pyruvate kinase by fructose-1,6-diphosphate was introduced into the model to allow glycolysis to provide the rate of ATP production required during muscle load activation.