Optimizing Ultrasensitive RT‐QuiC assay on AD tau seed‐monomer reactions

Upon optimization, the RT-QuiC assay is both ultrasensitive and specific to AD tau fibrillization. Our findings offer insights into the fibrillization mechanism of tau aggregates and expand our understanding of pathogenic tau seeds in AD. By optimizing the assay to more accessible patient samples in the future, namely CSF and plasma, the RT-QuiC can serve as a potent diagnostic tool for AD tau progressions.