Isolation of single neuronal nuclei by phospho‐tau accumulation in Alzheimer’s disease brain
We developed a highly efficient method using fluorescence-activated nuclear sorting (FANS) to separate the population of P-tau+ neuronal nuclei in AD brains. This method allows interrogation of human neuronal nuclei based on single-cell tau pathology and enables single-cell genomic studies of heterogeneous tau pathology in AD, toward insight into disease pathogenesis and therapeutic targets.
Authors
- Michael B. Miller (ORCID: https://orcid.org/0000-0002-6205-3314)
- Matthew P. Frosch (ORCID: https://orcid.org/0000-0002-3940-9861)
- Samantha L. Kirkham (ORCID: https://orcid.org/0000-0002-5444-6636)
- August Yue Huang (ORCID: https://orcid.org/0000-0002-0416-2854)
- Xuyu Qian (ORCID: https://orcid.org/0000-0001-5944-3816)
- Zinan Zhou (ORCID: https://orcid.org/0000-0003-2014-3650)
- Derek H. Oakley (ORCID: https://orcid.org/0000-0002-6998-9510)
- Bradley T. Hyman (ORCID: https://orcid.org/0000-0002-7959-9401)
- Bowen Jin (ORCID: https://orcid.org/0000-0002-9958-5824)
- S. T. Naik
Institutions
- Brigham and Women's Hospital (US)
- Boston Children's Hospital (US)
- Harvard University (US)
- Massachusetts General Hospital (US)
- MaineGeneral Medical Center (US)
Publication Details
- Journal
- Alzheimer s & Dementia
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1002/alz.093457
- Primary Topic
- Alzheimer's disease research and treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00