Applying Digital Spatial Profiling of the Transcriptome to Elucidate Disease Mechanisms of Psychosis in Alzheimer’s disease
This pilot study demonstrates the potential of the NanoString GeoMx™ DSP platform as an innovative spatial transcriptomics methodology for investigating AD+P with the potential to uncover differentially expressed genes that may be missed by bulk RNA sequencing studies. FFPE sections appear to be optimal. Analysing earlier stage disease and more sections per subject may help with better differentiation of tau and non-tau ROIs.
Authors
- Ehsan Pishva (ORCID: https://orcid.org/0000-0002-8964-0682)
- Michael Eyres (ORCID: https://orcid.org/0000-0002-9021-3901)
- Eleanor Platt
- Lucy R. Frost (ORCID: https://orcid.org/0000-0003-0750-778X)
- Luke Weymouth (ORCID: https://orcid.org/0000-0002-1168-6015)
- Katie Lunnon (ORCID: https://orcid.org/0000-0001-7570-6065)
- Adam R. Smith (ORCID: https://orcid.org/0000-0001-6495-2711)
- Gayle Marshall (ORCID: https://orcid.org/0000-0002-5839-1150)
- Byron Creese (ORCID: https://orcid.org/0000-0001-6490-6037)
- Jacob Thomas‐Hegarty
Institutions
- University of Exeter (GB)
- Maastricht University (NL)
- Medicines Discovery Catapult (GB)
- Brunel University of London (GB)
Publication Details
- Journal
- Alzheimer s & Dementia
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1002/alz.086517
- Primary Topic
- Health, Environment, Cognitive Aging
- Type
- article
- Field-Weighted Citation Impact
- 0.00