Production and characterization of human tau protein with site‐specific phosphorylation utilizing <i>E. coli</i> with an expanded genetic code

The ability to readily produce recombinant human tau with known PTMs will be a critical tool in helping researchers develop, validate, and assess the specificity of p-tau detection platforms and antibody-based technologies. With the idea of developmentally significant PTM sites in mind, we have preliminary results suggesting that even single tau PTMs can dramatically alter tau biochemistry and lead to disease-like molecular behaviors.