Gold Nanoparticles - Antibody Based Immunosensor for Detection of Arsenic in Ground Water Sample

The arsenic in groundwater samples is found to harm human health and the environment. Detection of arsenic by gold nanoparticle-antibody based immunosensor plays a vital role in the present day scenario where people need not use sophisticated laboratory detection systems if the efficient immunosensors are developed against metal contaminants. Gold nanoparticles (AuNPs) are chosen because they exhibit unique optical and electronic properties, which in-turn enhances the sensitivity, selectivity and specificity in the field of immunobiosensors. The design of the immunosensor involves conjugation of antibodies that are generated against arsenite (As³) by functionalizing with gold nanoparticles that helps in specific binding to arsenite (As³) in real samples. The concentration of arsenite (As³) under test was ranging from 50ng/l to 500ng/l. The dectection limit of arsenite (As³) was found to be 38ng/l by the designed gold nanoparticle-antibody based immunosensor.