LC-MS-Based Metabolite Profiling and Antioxidant Capacity Assessment of Ipomoea eriocarpa Extract

The objective of the research was to investigate the phytochemical analysis, total phenolic, total flavonoid, total alkaloid, in-vitro antioxidant profile and LC-MS analysis of Ipomoea eriocarpa whole plant extract. The process of extraction took place utilizing a Soxhlet apparatus with a hydroalcoholic solvent. An examination of qualitative phytochemicals showed the existence of phenolic, alkaloids, flavonoids, triterpenes, tannins, and unsaturated steroids The study determined the quantities of total phenolics, total flavonoids, and total alkaloids present were 94.6 ± 1.7 mg gallic acid equivalent (GAE)/g sample, 81.25 ± 2.2 mg quercetin equivalent/g, 44.32 ± 2.8 mg atropine equivalent/g. The evaluation of antioxidant properties was conducted using multiple methods, including DPPH assay, ferric reducing-antioxidant power (FRAP), reactive nitrogen oxide, and hydroxyl free radical scavenging method. The IC50 values in DPPH assay of standard (ascorbic acid) and sample were found to be 14.04 ± 0.02 and 36.12 ± 0.11 and FRAP scavenging assay of standard (ascorbic acid) and sample were found to be 3.167 ± 0.02 and 1.548 ± 2.75 respectively. The LC-MS (ES+) analysis of extract reported the presence of twelve phytoconstituents along with seven phytoconstituents in LC-MS (ES-). The study concluded that the I. eriocarpa hydroalcoholic extract possess antioxidant properties due to the existence of phenols and flavonoids and thus can be a valuable agent to prevent the development of various diseases.