Formulation Development and <i>In Vitro</i> Characterization of <i>Gymnema sylvestre</i>’s Phytochemical Encapsulated Phytosome

Background: Drug therapy for Type II diabetes mellitus suffers from a frequent administration of oral anti-diabetic drugs, which leads to drug tolerance and nephrotoxicity as major side effects. Aim: To address this issue, the current research focused on investigating gymnemic acid and its subsequent encapsulation in phytosomes on the release properties of anti-diabetic drugs. In this research work, gymnemic acid shall be encapsulated in a phospholipid bilayer, it is called a Phytosome and it will be thoroughly characterized through in vitro study. Methods: Phytosomes with gynemic acid encapsulation have to be created using the solvent evaporation method. In order to preserve their stability, the created phytosomes were lyophilized. Then the gymnemic acid encapsulated phytosome was characterized for Measurement of Zeta potential, Measurement of particle size, Determination of entrapment efficiency, Scanning electron microscopy, Drug content and In-vitro Drug release study. Results: The drug content and entrapment efficiency of the best formulation (F3) was found to be 43.41% and 78.43%. Conclusion: The study’s findings indicate that gymnemic acid may release the medication for a longer period and significantly lower blood glucose levels after being encapsulated into phytosomes.