Evaluation Of Secondary Metabolites From FicusbenjaminaAnd FicuskrishnaeThrough RP-HPTLCAnalysis

Over centuries, a plethora of plant-derived compounds have served as pivotal elements in traditional medicinal practices, persisting as indispensable constituents in contemporary therapeutic approaches.Notably, phytochemical constituents such as flavonoids, polyphenols, and alkaloids, abundantly present across diverse botanical species, have garnered substantial scientific interest owing to their purported health-promoting attributes.Current investigations underscore their potential therapeutic efficacy, encompassing anti-inflammatory, antioxidant, and anticancer properties, thereby illuminating their significance in the realm of modern pharmacotherapy.Through the integration of diverse methodologies including green chemistry practices, plant manipulation techniques, bioconversion processes, and ongoing advancements in natural product exploration, researchers stand poised to surmount the hurdles inherent in sourcing medicinal compounds from botanical reservoirs.This multifaceted approach holds promise in unraveling the comprehensive therapeutic properties inherent in plant-derived constituents, thereby facilitating the realization of their maximal therapeutic efficacy.Ficus benjamina and Ficus krishnae have garnered attention for their diverse pharmacological activities, contributing to the maintenance of overall well-being.The present research has been conducted to check the presence of quercetin and rutin quantitatively, in the hydroalcoholic extract of leaves of Ficus benjamina and Ficus krishnae by using HPTLC analysis.Quantitative estimation data so obtained have resulted in a good resolution from other constituents of the plant materials.The method provides rapid, simple, precise, specific, and accurate results.The method employs TLC plates made up of adsorbent material like silica gel and pre-coated with silica gel 60 F254, on which each sample was applied separately.The application dosage speed was 150nl/s.CAMAG TLC scanner III was utilized for scanning at 254 nm and 366 nm for quercetin and rutinrespectively.Superimposing spectra obtained from UV of the standard and sample within the same Rf confirmed the presence of constituents in the extracts i.e.Rf 0.31 and 0.73windows for Quercetin and Rutin respectively.