Effects of DNA methyltransferase 2 (DNMT2) on gastric cancer cells proliferation and migration via regulation of structural maintenance of chromosomes 3 (SMC3)

Background: DNA methyltransferase 2 (DNMT2) is reported as an RNA modifier regulating the expression of oncogenes in cancers.This work explored the regulatory potential of DNMT2 in gastric cancer (GC) cell proliferation and migration.Materials and Methods: GC cells were induced with Transforming growth factor-beta1 (TGF-1) for constructing DNMT2 overexpression model.GC cell proliferation was subject to Cell Counting Kit-8 (CCK-8) assay.GC cell migration was observed through wound scratch together with transwell migration assays.Results: The outcomes showed that the DNMT2 overexpression model was successfully built.Relative to the control, the levels of DNMT2 and SMC3 were apparently decreased by TGF-1 stimulation, whereas E-cadherin, Smad2 as well as Vimentin expression was elevated by TGF-1, and GC cell proliferation along with migration were significantly elevated.However, in comparison with the NC group, the DNMT2 overexpression group exhibited higher levels of DNMT2 and SMC3, significantly suppressed E-cadherin, Vimentin along with Smad2 expression, and significantly suppressed GC cell proliferation along with migration.Conclusion: Overexpression of DNMT2 inhibits GC cell proliferation along with migration.The level of SMC3 was also elevated by DNMT2 overexpression in GC cells.The findings of our study might provide the theoretical basis for the development of GC.