Use auxins and Antioxidants to Optimise Nutrient Medium of Tomato Tissue Culture

Abstract Calli characteristics of tomato grown in vitro on different nutrient media supplemented with auxins and antioxidants in various combinations were studied. Explants were cut from the hypocotyl, apex, and cotyledons and cultivated on Murashige and Skoog medium supplemented with some auxins and antioxidants. It was found that the regeneration potential was higher in combinations with joint application of antioxidant and a phytohormone of the auxin group — indolyl-acetic acid. The obtained results demonstrate differential influence of specific biologically active substances on the manifestation of calli characteristics depending on the type of explant.