Development and Characterization of a Magnesium Membrane Loaded with Hyaluronic Acid, Tricalcium Phosphate, and Quercetin for Bone Healing Applications

This study aims to develop and characterize a hyaluronic acid/Tricalcium Phosphate (TCP)/quercetin-doped magnesium membrane for Guided Bone Regeneration (GBR), targeting applications in periodontal and other biomedical fields.The goal is to create a biocompatible, hydrophilic membrane with enhanced properties suitable for promoting bone regeneration.A polymeric solution containing TCP, PVA, hyaluronic acid, and quercetin-doped magnesium nanoparticles was electrospun to create nanofibrous membranes.These membranes were analyzed using FTIR for chemical interactions, XRD for nanoparticle distribution, SEM for morphology, and water contact angle measurements for hydrophilicity.Cell viability was assessed with an MTT assay using Dental Pulp Stem Cells, and bone formation potential was evaluated using MG63 and osteoclast cells with Alizarin Red staining.The fabricated membranes demonstrated significant hydrophilicity, which is critical for GBR applications.SEM analysis revealed a nanofibrous structure with appropriate pore size, facilitating cell attachment and growth.The FTIR confirmed the expected chemical bonding, while XRD verified the incorporation of magnesium-doped nanoparticles.MTT assays showed high cell viability, indicating good biocompatibility.Furthermore, the bone formation assay confirmed the membrane's potential to support osteogenesis.These findings suggest that the hyaluronic acid/TCP/quercetin-doped magnesium membranes developed in this study exhibit favorable properties for use in guided bone regeneration, offering promising potential for addressing the limitations of current periodontal treatments and improving patient outcomes.