Rheology and Morphological Study of Anaemic Red Blood Cell Parameters Irradiated with Blue and Green Laser Beam

Numerous studies have explored the effects of low-level laser therapy (LLLT) on blood parameters over the past decade. This study investigates the in vitro effects of blue and green laser light on the rheological properties and morphology of anemic human red blood cells (RBCs). A total of 36 blood samples were collected and analyzed before and after laser irradiation at exposure times of 30, 60, and 90 seconds. Key blood parameters, including hematocrit (HCT), mean cell volume (MCV), and mean corpuscular hemoglobin (MCH), were measured immediately after irradiation using a hematology analyzer, followed by morphological evaluation. Significant changes were observed in HCT and MCV for both blue and green laser irradiation. Notably, RBC deformability increased, particularly after 90 seconds of laser exposure. The findings demonstrate that anemic samples are more affected by laser irradiation than normal samples, with green laser showing a greater impact on RBC morphology compared to blue laser