Interfacial adaptation and ion release of bioceramic sealer in infectious environment

This work used energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM) to evaluate and compare the ion release and interfacial adaptability of two bioceramic sealants (Dia-Root and One-Fil sealers) under standard circumstances (PBS) and in infectious environments. Methods: Eighty dentin slices with standardized canal spaces were divided into two groups (n = 40 each) and sealed with Dia-Root and One-Fil sealants. Each set was partitioned into two subgroups (n = 20) based on the experimental liquids: (a) PBS (pH 7.4) and (b) 1 mmol/L butyric acid (pH 5.4). The specimens were then cultured for seven days at 37 degrees Celsius. The ion release was examined utilizing EDX. Scanning Electron Microscopy (SEM) will be employed to examine the marginal gap at the contact between the sealer and root dentin. Subsequently, a statistical analysis of the collected values was conducted. Outcomes: One-Fil bioceramic sealant released a greater quantity of calcium ions than Dia-Root BIO Sealer when subjected to acid treatment; nonetheless, the difference between the sealers was not statistically significant. Phosphorus was identified in all sealing specimens exposed to PBS, but not in those submitted to butyric acid. Moreover, the EDX analysis of the evaluated sealers exposed to PBS revealed mean Ca/P ratios exceeding the natural hydroxyapatite (HAP) ratio of 1.67. No statistically significant change was seen in the gap width between the tested sealants exposed to acid and dentin (P > 0.05). The mean and standard deviation for Dia-Root and One-Fil bioceramic sealants subjected to PBS were calculated, revealing that Dia-Root BIO Sealer had the smallest gap, whilst One-Fil exhibited the biggest gap. Conclusions: All analyzed sealers (Dia-Root and One-Fil) generate calcium, aluminum, and oxygen ions across all storage media. Acidic environments affect the interfacial adaptation of the evaluated bioceramic sealers to root canal dentin, as the statistically significant mean gap width of all tested sealers exposed to butyric acid was larger than that of the same sealers exposed to PBS.