An Attempt to Reduce Carbon Footprint by Enhancing the Synthesis of Biofuels with Pichia Pastoris

Biofuels being renewable sources of energy means that they are cleaner, more sustainable and reduce greenhouse gas emissions and reliance on fossil fuel intake. The application of Pichia pastoris and its role as an enhancer in the industrial synthesis of alternative fuels in comparison with the traditional systems containing Escherichia coli, has shown promising results. This paper reviews the synthesis of biofuels with P.pastoris, an integrated approach used for enhancing the production of lipases which catalyze the process. P. pastoris enables the expression in a eukaryotic system, suitable for the synthesis of high-activity and glycosylated enzymes that can work more stably and efficiently under industrial conditions. This process also obtains useful by-products such as glycerol and long chain fatty acids, which find applications in pharmaceuticals, cosmetics, and food industries adding economic value to the industry and filling the concept of a circular bioeconomy model. The use of P. pastoris in biofuel production significantly reduces the carbon footprint by using feedstocks that absorb carbon dioxide from the atmosphere. When these biofuels are burnt, they release carbon dioxide that was recently captured from the environment creating a closed carbon cycle. Advances in biofuel production using P. pastoris have demonstrated its superior efficiency as a platform for large-scale biofuel production and the generation of high-value biochemicals. These results indicate that P. pastoris has significant potential for producing environmentally friendly and economically viable biofuels.