Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells
<b>Background:</b> Energy delivered at different wavelengths causes different types of damage to DNA. <b>Methods:</b> PC-3, FaDu, 4T1 and B16-F10 cells were irradiated with different wavelengths of ultraviolet light (UVA/UVC) and ionizing radiation (X-ray). Furthermore, different photosensitizers (ortho-iodo-Hoechst33258/psoralen/trioxsalen) were tested for their amplifying effect. Survival fraction and damage analysis using the γH2A.X assay (double-strand breaks) and the ELISA assay (cyclobutane pyrimidine dimers) were compared. <b>Results:</b> The PC-3 cells were found to be the most sensitive cells to the treatment strategies used. FaDu and PC-3 showed a strong sensitivity to UVA. Analysis of the damage showed that the cell lines exhibited different sensitivities. <b>Conclusions:</b> Thus, an enhancing effect of photosensitizers (PS) in combination with UVA could be demonstrated in some cases. However, this is cell- and dose-dependent.
Authors
- Ralph A. Bundschuh (ORCID: https://orcid.org/0000-0001-7291-9372)
- Katja Tietze
- Florian Brandt (ORCID: https://orcid.org/0000-0001-7711-4805)
- Marc Pretze (ORCID: https://orcid.org/0000-0002-6432-5694)
- Lisa Hübinger
- Jörg Kotzerke
- K. Wetzig
Institutions
- University Hospital Carl Gustav Carus (DE)
- Technische Universität Dresden (DE)
Publication Details
- Journal
- Biomedicines
- Published
- 2024-12-31
- DOI
- https://doi.org/10.3390/biomedicines13010073
- Primary Topic
- Photodynamic Therapy Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00