Genome-Wide Identification and Expression Profiling of the SPL Transcription Factor Family in Response to Abiotic Stress in Centipedegrass

SQUAMOSA promoter-binding protein-like (SPL) transcription factors play a critical role in the regulation of gene expression and are indispensable in orchestrating plant growth and development while also improving resistance to environmental stressors. Although it has been identified across a wide array of plant species, there have been no comprehensive studies on the <i>SPL</i> gene family in centipedegrass [<i>Eremochloa ophiuroides</i> (Munro) Hack.], which is an important warm-season perennial C4 turfgrass. In this study, 19 potential <i>EoSPL</i> genes in centipedegrass were identified and assigned the names <i>EoSPL1</i>-<i>EoSPL19</i>. Gene structure and motif analysis demonstrated that there was relative consistency among the branches of the phylogenetic tree. Five pairs of segmental duplication events were detected within centipedegrass. Ten <i>EoSPL</i> genes were predicted to be targeted by miR156. Additionally, the <i>EoSPL</i> genes were found to be predominantly expressed in leaves and demonstrated diverse responses to abiotic stress (salt, drought, glufosinate ammonium, aluminum, and cold). This study offers a comprehensive insight into the <i>SPL</i> gene family in centipedegrass, creating a foundation for elucidating the functions of <i>EoSPL</i> genes and investigating their involvement in abiotic stress responses.