Effects of Platelets Rich Plasma and N Acetyl Cysteine on Achilles Tendon Regeneration in Dog

Background: The Achilles tendon is one of the most important tendons in the body. When any problem or defect occurs, such as (cut, tear or puncture), it may hinder the motor functions of the foot. The recent study highlights the effective role of N_acetyl cysteine and Platelet_rich plasma in accelerating the healing process of the tendon. Aim of the Study: The present study aimed to study and compare the effects of Platelets rich plasma and N acetyl cysteine on Achilles tendon regeneration after tendon defect inducing in dog. Materials and Methods: Fifteen adult dogs were utilized in this study , the dogs were divided into three groups(5 dogs for each one): Group A(PRP group) was treated with PRP topically on the tendon, Group B(N-AC group) in which tendon defect was treatedby topical application of N-acetylcysteine , while in Group C(control group ) the tendon defect was left without treatment.The animal was aneshtetized and the right hind limb was prepared for aspetic surgery , Achilles tendon defect was created by using needle 18 guage (core lesion) and the site of operation was closed routinally Achilles tendon healing and histopathological monitoring were evaluated at 42 days post-injury. Results:Throughout the study, the participants' general health was assessed daily. Results showed that tendon healing was more successful in the PRP- and NAC-treated tendon than in the untreated tendon, with significant improvement in the treated groups after 42 days. Discussion: Our study has shown that utilizing PRP and NAC for treating tendons is successful in preserving certain structural characteristics during the initial stage of tendon remodeling due to the growth factors in PRP and the NAC's capability in assisting TSPCs to survive and differentiate helps with tendon recovery after damage.